pcr arrays Search Results


digital pcr  (fluidigm)
92
fluidigm digital pcr
Digital Pcr, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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drug transporter quantitative reverse transcriptase pcr array  (Qiagen)
96
Qiagen drug transporter quantitative reverse transcriptase pcr array
Drug Transporter Quantitative Reverse Transcriptase Pcr Array, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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digital array ifc  (fluidigm)
94
fluidigm digital array ifc
Digital Array Ifc, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative reverse transcriptase pcr array analysis rt 2 profiler pcr array plates  (Qiagen)
96
Qiagen quantitative reverse transcriptase pcr array analysis rt 2 profiler pcr array plates
Quantitative Reverse Transcriptase Pcr Array Analysis Rt 2 Profiler Pcr Array Plates, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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real time reverse transcriptase pcr array  (Qiagen)
96
Qiagen real time reverse transcriptase pcr array
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
Real Time Reverse Transcriptase Pcr Array, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr array 181 rneasy mini kit  (Qiagen)
96
Qiagen pcr array 181 rneasy mini kit
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
Pcr Array 181 Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atherosclerosis rt2 profiler pcr arrays  (Qiagen)
90
Qiagen atherosclerosis rt2 profiler pcr arrays
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
Atherosclerosis Rt2 Profiler Pcr Arrays, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat cell death pathfinder rt2 profilertm pcr array  (Qiagen)
90
Qiagen rat cell death pathfinder rt2 profilertm pcr array
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
Rat Cell Death Pathfinder Rt2 Profilertm Pcr Array, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt2 profiler pcr array rat endothelial cell biology  (Qiagen)
90
Qiagen rt2 profiler pcr array rat endothelial cell biology
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
Rt2 Profiler Pcr Array Rat Endothelial Cell Biology, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miscript mirna pcr array rat serum & plasma rat plasma mirnome pcr plates (96 wells)  (Qiagen)
90
Qiagen miscript mirna pcr array rat serum & plasma rat plasma mirnome pcr plates (96 wells)
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
Miscript Mirna Pcr Array Rat Serum & Plasma Rat Plasma Mirnome Pcr Plates (96 Wells), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt2 profilertm pcr array human nephrotoxicity  (Qiagen)
90
Qiagen rt2 profilertm pcr array human nephrotoxicity
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
Rt2 Profilertm Pcr Array Human Nephrotoxicity, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative pcr array plates qiagen pamm-067z  (Qiagen)
90
Qiagen quantitative pcr array plates qiagen pamm-067z
Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). <t>(B)</t> <t>RNA</t> was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time <t>RT-PCR</t> Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.
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Image Search Results


Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). (B) RNA was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time RT-PCR Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.

Journal: Cellular immunology

Article Title: Secretion of MIP-1? and MIP-1? by CD8 + T-lymphocytes Correlates with HIV-1 Inhibition Independent of Coreceptor Usage

doi: 10.1016/j.cellimm.2010.09.011

Figure Lengend Snippet: Histone hyperacetylation regulates the suppressive ability of CD8+ T-lymphocytes and expression of cytokines. (A) CD8+ T-lymphocytes were cultured for 16 h in growth media supplemented with 2.0 mM valproic acid (filled bars) or PBS (open bars) as a vehicle control. Subsequently, the CD8+ T-lymphocytes were used in a direct contact noncytolytic CD8 suppression assay. Mean log10 reduction in HIV-1 replication and standard error are shown. Significance was determined with a Student’s t-test (* p < 0.05; ** p < 0.01). (B) RNA was isolated from the valproic acid-treated or control CD8+ T-lymphocytes used in (A), and differences in gene expression of 164 cytokine and cytokine receptor genes were analyzed by real-time RT-PCR Array. Mean fold change in expression of each gene is displayed, with negative fold changes designating down-regulated genes, and positive fold changes signifying genes upregulated in valproic acid-treated cells compared to control cells. Genes within the area shaded in gray were considered unchanged (less than a 3-fold change in expression). Fold down-regulation of genes in JR-HVS (C) and VC18 (D) CD8+ T-lymphocytes after inhibition of histone deacetylation.

Article Snippet: Total RNA was extracted from the remaining treated cells to be analyzed by Real-time Reverse transcriptase-PCR Array (SA Biosciences).

Techniques: Expressing, Cell Culture, Suppression Assay, Isolation, Quantitative RT-PCR, Inhibition